Basic Microbiological Techniques

• Explain the term ‘microbiological media’ and name the three different media forms
• Explain the difference between agar plates, agar slants and agar deeps
• Explain the difference between reserve and working stock cultures
• Describe how reserve and working stock cultures are used and stored
• Describe the quadrant and continuous streak plate methods
• Describe the common streak plate method mistakes
• Describe the pour plate method

Learner Benefits:

• Foundational Knowledge in Media Preparation: Understand the different types of microbiological media and the correct procedures for preparing each form, from liquid broths to solid and semisolid agar, to support optimal microbial growth.

• Competence in Handling and Managing Stock Cultures: Learn the differences between reserve and working stock cultures, their purposes, and proper storage methods to maintain culture viability and prevent contamination.

• Proficiency in Pure Culture Techniques: Master the quadrant and continuous streak methods, critical for isolating pure bacterial colonies, while recognizing and avoiding common errors that can compromise results.

• Enhanced Practical Skills in Laboratory Procedures: Gain confidence in executing media sterilization, streak plate techniques, and the pour plate method, with a strong emphasis on aseptic technique and best practices for microbial laboratory work.

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