Basic Microbiological Techniques
An overview of essential microbiological techniques frequently performed in laboratory settings, emphasizing the preparation of different types of media, proper handling and storage of stock cultures, and methods for obtaining pure cultures. The module covers media forms (liquid, solid, and semisolid), detailed steps for preparing agar plates, deeps, and slants, as well as critical techniques like quadrant and continuous streak plating and the pour plate method. Additionally, it highlights common mistakes and best practices to ensure successful microbial culture outcomes.
Whether for onboarding or annual refresher training, this Module is seamlessly deployable on any LMS and can be tailored to your company’s exact needs.
Duration: 30 Mins
Grade: Core Library
Description
- Foundational Knowledge in Media Preparation: Understand the different types of microbiological media and the correct procedures for preparing each form, from liquid broths to solid and semisolid agar, to support optimal microbial growth.
- Competence in Handling and Managing Stock Cultures: Learn the differences between reserve and working stock cultures, their purposes, and proper storage methods to maintain culture viability and prevent contamination.
- Proficiency in Pure Culture Techniques: Master the quadrant and continuous streak methods, critical for isolating pure bacterial colonies, while recognizing and avoiding common errors that can compromise results.
- Enhanced Practical Skills in Laboratory Procedures: Gain confidence in executing media sterilization, streak plate techniques, and the pour plate method, with a strong emphasis on aseptic technique and best practices for microbial laboratory work.
Learning Objectives
- Explain the term ‘microbiological media’ and name the three different media forms.
- Explain the difference between agar plates, agar slants and agar deeps.
- Explain the difference between reserve and working stock cultures.
- Describe how reserve and working stock cultures are used and stored.
- Describe the quadrant and continuous streak plate methods.
- Describe the common streak plate method mistakes.
- Describe the pour plate method.
Keywords
- Agar Plate Methods
- Aseptic Technique
- Autoclaving
- Contamination Prevention
- Continuous Streak Method
- Laboratory Techniques
- Microbiological Media Preparation
- Pour Plate Method
- Pure Culture Techniques
- Quadrant Streak Method
- Stock Culture Management
Module Features
Animations
Voice Over
Knowledge Checks
Assessments
SCORM/AICC compatible
Full Screen
