Downstream Processing: Ultrafiltration and Diafiltration

• Explain the role of filtration in protein purification methods
• Describe the role of ultrafiltration / diafiltration (UF/DF) in downstream processing
• Describe the difference between Normal Flow Filtration (NFF) and Tangential Flow Filtration (TFF)
• List the advantages of TFF over NFF
• Explain the terms molecular weight cut off and transmembrane pressure
• Identify important components of a UF/DF skid and their functions
• List and explain the critical process parameters involved in a UF/DF operation
• Describe the in-process checks and quality tests that are carried out during a UF/DF operation
• Describe how a UF/DF skid is cleaned
• Describe the safety requirements associated with UF/DF
• List some common problems encountered when operating UF/DF

Learner Benefits:

• Gain in-depth knowledge of the role of ultrafiltration and diafiltration in protein purification, including the differences and advantages of normal flow and tangential flow filtration.

• Enhance your technical vocabulary and understanding of key terminology such as molecular weight cut-off, transmembrane pressure, retentate, and permeate.

• Explore the components of UF/DF skids and their functions.

• Identify critical process parameters and understand the role of in-process checks, quality tests, and troubleshooting during UF/DF operations.

• Gain practical knowledge of the set-up, operation, and cleaning of ultrafiltration and diafiltration systems.

• Understand the safety requirements associated with UF/DF operations and learn how to maintain high standards of cleanliness and integrity in biopharmaceutical manufacturing environments.

    Animations
   Voice Over
    Knowledge Checks
    Assessments
   SCORM/AICC compatible
    Full Screen