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Founded in 1998 in Dublin, Ireland, Skillpad's team of industry-experienced Subject Matter Experts and Consultants believes Pharma, Biopharma, and Life Science Industry manufacturing companies can achieve production excellence with improved performance, productivity & compliance, as well as reduced losses & down-time, if they have KNOWLEDGE CONSISTENCY in all critical areas of manufacturing operations.

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    HPLC Troubleshooting

    Describes the troubleshooting of common HPLC equipment problems. Examples include leaks, column frit blockages, baseline noise and detector malfunctions. The Module also describes the approach to troubleshooting these commonly encountered problems, and will be an invaluable resource to anyone working with HPLC on a regular basis.

    Whether for onboarding or annual refresher training, this Module is seamlessly deployable on any LMS and can be tailored to your company’s exact needs.

      Duration: 30 Mins

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    SKU: PPL-1131-SPL01-EN Category: Tag:

    Description

    Learning Objectives:

    • Explain why proper sample preparation is critical in HPLC and why degassing is performed
    • Explain how injector and needle performance can affect a HPLC analysis
    • Explain why HPLC system pumps must be calibrated
    • Describe the problems associated with pressure being too high or too low in HPLC and how these problems can be resolved
    • Explain the purpose of HPLC System Suitability testing
    • Describe the most common problems affecting baseline, peak shape, and detectors, and how they can be resolved
    • List the areas of a HPLC system that can be affected by leaks
    • Describe the best practices for care of a HPLC column

    Learner Benefits:

    • Master Sample Preparation Techniques: Understand the critical role of complete dissolution, filtration, and degassing in ensuring accurate and reliable HPLC results, and learn best practices to prevent contamination and interference.
    • Injector and Needle Optimization: Gain practical knowledge of how proper maintenance and blank injections can safeguard against performance issues, ensuring precise sample delivery.
    • Pump Calibration and Pressure Management: Learn why regular pump calibration is crucial and acquire strategies for identifying and resolving both high and low-pressure problems, ensuring stable flow and optimal system performance.
    • System Suitability Testing and Troubleshooting: Familiarization with the purpose of system suitability tests and how to interpret retention time shifts, resolve baseline noise, and troubleshoot column issues for better separation and peak resolution.
    • Detect and Prevent Leaks and System Failures: Identify areas prone to leaks, such as columns, pumps, and detectors, and learn effective solutions to maintain the integrity of high-pressure connections.
    • Prolong Column Life Through Best Practices: Discover proper storage and handling techniques to extend the lifespan of HPLC columns, minimizing the need for replacements and ensuring consistent, high-quality results over time.
    		 Module Features:

        Animations
       Voice Over
        Knowledge Checks
        Assessments
       SCORM/AICC compatible
        Full Screen

    Keywords

    • Baseline Stability
    • Column Maintenance
    • Degassing
    • Detector Issues
    • Dissolution
    • Filtration
    • High Pressure
    • HPLC Troubleshooting
    • Injector Performance
    • Low Pressure
    • Needle Maintenance
    • Peak Shape
    • Pump Calibration
    • Retention Time
    • Sample Preparation

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