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Founded in 1998 in Dublin, Ireland, Skillpad's team of industry-experienced Subject Matter Experts and Consultants believes Pharma, Biopharma, and Life Science Industry manufacturing companies can achieve production excellence with improved performance, productivity & compliance, as well as reduced losses & down-time, if they have KNOWLEDGE CONSISTENCY in all critical areas of manufacturing operations.

With 25 years in business, and offices in Europe, the USA and Canada, Skillpad has helped customers all over the world with Life Science Industry Knowledge challenges. We are fully dedicated to the idea that Knowledge Consistency is more achievable than ever using the latest technology and advanced knowledge transfer and management tools.

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    Library

    HPLC Troubleshooting

    A comprehensive guide to efficiently diagnosing and resolving issues in High-Performance Liquid Chromatography (HPLC) systems. The module covers essential areas such as sample preparation, injector and needle performance, pump calibration, pressure inconsistencies, and column and detector maintenance. Learners will gain insights into minimizing disruptions and maintaining the accuracy and reliability of HPLC analyses, with clear explanations of preventative measures and troubleshooting techniques for common issues affecting baseline stability, peak shapes, and overall system performance.

    Whether for onboarding or annual refresher training, this Module is seamlessly deployable on any LMS and can be tailored to your company’s exact needs.

      Duration: 30 Mins
    Grade: Premium Performance

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    SKU: PPL-1131-SPL01-EN Category: Tag:

    Description

    • Master Sample Preparation Techniques: Understand the critical role of complete dissolution, filtration, and degassing in ensuring accurate and reliable HPLC results, and learn best practices to prevent contamination and interference.
    • Injector and Needle Optimization: Gain practical knowledge of how proper maintenance and blank injections can safeguard against performance issues, ensuring precise sample delivery.
    • Pump Calibration and Pressure Management: Learn why regular pump calibration is crucial and acquire strategies for identifying and resolving both high and low-pressure problems, ensuring stable flow and optimal system performance.
    • System Suitability Testing and Troubleshooting: Familiarization with the purpose of system suitability tests and how to interpret retention time shifts, resolve baseline noise, and troubleshoot column issues for better separation and peak resolution.
    • Detect and Prevent Leaks and System Failures: Identify areas prone to leaks, such as columns, pumps, and detectors, and learn effective solutions to maintain the integrity of high-pressure connections.
    • Prolong Column Life Through Best Practices: Discover proper storage and handling techniques to extend the lifespan of HPLC columns, minimizing the need for replacements and ensuring consistent, high-quality results over time.

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