Introduction to HPLC
Explore the fundamentals of High-Performance Liquid Chromatography (HPLC), the essential technique used in pharmaceutical quality control for the identification and quantification of compounds. This module covers key concepts, including the theory behind HPLC, its components, and the differences between reverse phase and normal phase chromatography. Gain insights into the separation process, the significance of polarity, and how various factors influence analyte retention, peak area, and resolution. The content emphasizes practical understanding, including HPLC system setup, sample injection methods, and interpretation of results.
Whether for onboarding or annual refresher training, this Module is seamlessly deployable on any LMS and can be tailored to your company’s exact needs.
 Duration: 30 Mins
Grade: Premium Performance
Description
- Foundational Understanding of HPLC Theory: Grasp the principles behind HPLC and understand how chromatography separates and analyzes chemical compounds based on polarity and other physical properties.
- Familiarity with HPLC Components: Identify and comprehend the purpose and operation of key system components, such as the injector, pump, column, and detector, crucial for effective use of HPLC equipment.
- Insight into Chromatographic Phases: Differentiate between reverse phase and normal phase chromatography and understand how the mobile and stationary phases affect analyte separation.
- Optimization Skills: Learn how to manipulate separation parameters for better resolution and efficiency, and recognize factors influencing retention times and peak shape.
- Mastery of Detection and Quantification: Understand how to interpret HPLC results, construct standard curves, and calculate limits of detection and quantification, enhancing your ability to conduct precise and reliable analyses.
- Practical Applications in QC: Develop the expertise to apply HPLC techniques in quality control laboratories, ensuring the purity, potency, and safety of pharmaceutical products.
Learning Objectives
- Describe the components of a HPLC column.
- Explain the basic concepts behind chromatography.
- Describe the meaning of ‘partitioning’.
- Differentiate between a mobile phase and a stationary phase.
- Explain the components of a system diagram of a HPLC.
- Explain how phase polarity can assist separation.
- List the methods commonly employed for optimization of separation.
Keywords
- Analyte Separation
- Analytical Chromatography
- Analytical Method Optimization
- Column Efficiency
- High Performance Liquid Chromatography (HPLC)
- Limit of Detection (LOD)
- Limit of Quantification (LOQ)
- Mobile Phase
- Peak Resolution
- Pharmaceutical Quality Control
- Reverse Phase Chromatography
- Standard Curve
- UV-Visible Spectrophotometry
Module Features
  Animations
  Voice Over
  Knowledge Checks
  Assessments
  SCORM/AICC compatible
  Full Screen
