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    Introduction to HPLC

    Describes the basic theory and practice of HPLC. Particular focus is given to reverse phase HPLC. The components of a HPLC are described in detail and significant consideration is given to the explanation of results and peak shape. Analytical considerations of special relevance to HPLC are also presented.

    Whether for onboarding or annual refresher training, this Module is seamlessly deployable on any LMS and can be tailored to your company’s exact needs.

      Duration: 30 Mins

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    SKU: PPL-1130-SPL01-EN Category: Tag:

    Description

    Learning Objectives:

    • Describe the components of a HPLC column
    • Explain the basic concepts behind chromatography
    • Describe the meaning of ‘partitioning’
    • Differentiate between a mobile phase and a stationary phase
    • Explain the components of a system diagram of a HPLC
    • Explain how phase polarity can assist separation
    • List the methods commonly employed for optimization of separation

    Learner Benefits:

    • Foundational Understanding of HPLC Theory: Grasp the principles behind HPLC and understand how chromatography separates and analyzes chemical compounds based on polarity and other physical properties.
    • Familiarity with HPLC Components: Identify and comprehend the purpose and operation of key system components, such as the injector, pump, column, and detector, crucial for effective use of HPLC equipment.
    • Insight into Chromatographic Phases: Differentiate between reverse phase and normal phase chromatography and understand how the mobile and stationary phases affect analyte separation.
    • Optimization Skills: Learn how to manipulate separation parameters for better resolution and efficiency, and recognize factors influencing retention times and peak shape.
    • Mastery of Detection and Quantification: Understand how to interpret HPLC results, construct standard curves, and calculate limits of detection and quantification, enhancing your ability to conduct precise and reliable analyses.
    • Practical Applications in QC: Develop the expertise to apply HPLC techniques in quality control laboratories, ensuring the purity, potency, and safety of pharmaceutical products.
    		 Module Features:

        Animations
       Voice Over
        Knowledge Checks
        Assessments
       SCORM/AICC compatible
        Full Screen

    Keywords

    • Analyte Separation
    • Analytical Chromatography
    • Analytical Method Optimization
    • Column Efficiency
    • High Performance Liquid Chromatography (HPLC)
    • Limit of Detection (LOD)
    • Limit of Quantification (LOQ)
    • Mobile Phase
    • Peak Resolution
    • Pharmaceutical Quality Control
    • Reverse Phase Chromatography
    • Standard Curve
    • UV-Visible Spectrophotometry

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