Principles of Good Aseptic Technique

• Explain why good aseptic technique is necessary in the microbiological laboratory
• Explain the terms ‘false positive’ and ‘positive control’
• Describe the general steps for reducing the risk of contamination in microbiological testing
• List the three common steps that apply to all microbiological handling techniques
• Describe how to flame an inoculating loop
• Describe the correct aseptic technique for handling liquid broths
• Describe the correct aseptic technique for handling agar plates

Learner Benefits:

• Understanding Contamination Risks: Gain a deep appreciation for how microorganisms can impact microbiological testing and the necessity of rigorous aseptic practices to prevent contamination.

• Minimizing False Positives: Learn about the causes of false positives and acquire the skills to minimize these risks, thereby improving the reliability and accuracy of test results.

• Mastering Essential Techniques: Develop proficiency in core aseptic methods, such as sterilizing inoculating loops and handling liquid broths and agar plates, to uphold laboratory safety and test validity.

• Efficient Work Practices: Understand the importance of preparing your work area and materials in advance, minimizing potential contamination and ensuring efficient laboratory operations.

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