Principles of Good Aseptic Technique
The fundamentals of aseptic techniques are essential for microbiological testing in pharmaceutical and biologics environments. Learn how to minimize the risk of contamination through a structured approach to handling sterile media and performing microbial testing. The module covers key practices such as sterilizing inoculating loops, proper procedures for handling agar plates and liquid broths, and strategies to prevent false positive results. Detailed explanations emphasize the significance of maintaining sterile work areas, proper flaming techniques, and the use of positive and negative controls to ensure the integrity of microbiological results.
Whether for onboarding or annual refresher training, this Module is seamlessly deployable on any LMS and can be tailored to your company’s exact needs.
Duration: 30 Mins
Grade: Core Library
Description
- Understanding Contamination Risks: Gain a deep appreciation for how microorganisms can impact microbiological testing and the necessity of rigorous aseptic practices to prevent contamination.
- Minimizing False Positives: Learn about the causes of false positives and acquire the skills to minimize these risks, thereby improving the reliability and accuracy of test results.
- Mastering Essential Techniques: Develop proficiency in core aseptic methods, such as sterilizing inoculating loops and handling liquid broths and agar plates, to uphold laboratory safety and test validity.
- Efficient Work Practices: Understand the importance of preparing your work area and materials in advance, minimizing potential contamination and ensuring efficient laboratory operations.
Learning Objectives
- Explain why good aseptic technique is necessary in the microbiological laboratory.
- Explain the terms ‘false positive’ and ‘positive control’.
- Describe the general steps for reducing the risk of contamination in microbiological testing.
- List the three common steps that apply to all microbiological handling techniques.
- Describe how to flame an inoculating loop.
- Describe the correct aseptic technique for handling liquid broths.
- Describe the correct aseptic technique for handling agar plates.
Keywords
- Agar Plate Handling
- Aseptic Technique
- Bunsen Burner
- Contamination Prevention
- False Positives
- Good Manufacturing Practices (GMP)
- Inoculating Loop
- Laboratory Safety
- Microbiological Testing
Module Features
Animations
Voice Over
Knowledge Checks
Assessments
SCORM/AICC compatible
Full Screen
