Protein Purification in Downstream Processing
Describes the use of various chromatographic methods in downstream protein purification including size exclusion, ion exchange, hydrophobic interaction and affinity chromatographies. The basics of a chromatography set-up are covered along with critical factors affecting protein separation such as column packing, resolution, column capacity, pressure and the gel matrix.
Whether for onboarding or annual refresher training, this Module is seamlessly deployable on any LMS and can be tailored to your company’s exact needs.
 Duration: 30 Mins
Grade: Premium Performance
Description
- Develop a deep understanding of the entire protein purification process, focusing on the critical role that chromatography plays in downstream processing for both intracellular and extracellular proteins.
- Learn the principles and applications of different chromatographic methods, including ion exchange, hydrophobic interaction, size exclusion, and affinity chromatography, and how they contribute to high-resolution separation and product yield.
- Understand the factors affecting chromatography such as column packing, gel properties, and process parameters, and how these can be used to optimize the yield and purity of biopharmaceutical products.
- Build a robust technical vocabulary related to chromatography and protein purification, enhancing your ability to communicate effectively in this area.
Learning Objectives
- Describe a general outline of a protein purification process for both intracellular and extracellular proteins.
- Describe where chromatography fits into downstream processing.
- Explain the role of different chromatographies in protein purification.
- Describe the difference between Size Exclusion Chromatography, Ion-Exchange Chromatography, Hydrophobic Interaction Chromatography, and Affinity Chromatography.
- Explain what is meant by preparative chromatography.
- Explain terms such as solubility, charge, molecular size, adsorption properties and binding specificity.
Module Features
  Animations
  Voice Over
  Knowledge Checks
  Assessments
  SCORM/AICC compatible
  Full Screen
