Protein Purification in Downstream Processing

• Describe a general outline of a protein purification process for both intracellular and extracellular proteins
• Describe where chromatography fits into downstream processing
• Explain the role of different chromatographies in protein purification
• Describe the difference between Size Exclusion Chromatography, Ion-Exchange Chromatography, Hydrophobic Interaction Chromatography, and Affinity Chromatography
• Explain what is meant by preparative chromatography
• Explain terms such as solubility, charge, molecular size, adsorption properties and binding specificity

Learner Benefits:

• Develop a deep understanding of the entire protein purification process, focusing on the critical role that chromatography plays in downstream processing for both intracellular and extracellular proteins.

• Learn the principles and applications of different chromatographic methods, including ion exchange, hydrophobic interaction, size exclusion, and affinity chromatography, and how they contribute to high-resolution separation and product yield.

• Understand the factors affecting chromatography such as column packing, gel properties, and process parameters, and how these can be used to optimize the yield and purity of biopharmaceutical products.

• Build a robust technical vocabulary related to chromatography and protein purification, enhancing your ability to communicate effectively in this area.

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